m2 macrophage generation medium dxf (PromoCell)

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PromoCell m2 macrophage generation medium dxf

Study recap – recreating a pathological microenvironment on a kidney-on-a-chip microfluidic instrument to investigate the presence of a biological switch underlying renal calcification. A) Repurposing a proximal tubule-on-a-chip (kidney-on-a-chip) instrument for renal patient-derived <t>macrophage</t> M1 and <t>M2</t> proliferation on the top and bottom channels of the organ-chip instrument. B) a-c: The confluence of the macrophage cell lines was well supported on the organ chip instrument surface and on the tissue-culture plates: the cluster-forming cells are motile. Apoptotic assays were conducted on a chip and a plate to emulate bioinspired oxidative stress to observe the expression of the biomarkers of prolyl hydroxylase domain 2 (PHD2) and CellRox dye (orange and green, respectively). The morphology of the cells dramatically changed from miniature, motile M1 and M2 variants to aggregated cell clumps emitting PHD2 and CellRox under hypoxia [Jeewandara T et al. 2023].

M2 Macrophage Generation Medium Dxf, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m2+macrophage+generation+medium+dxf/M2-Macrophage+Generation+Medium+XF/bio_rxiv__2025__01__09__630316-95-13-18
Average 94 stars, based on 28 article reviews

m2 macrophage generation medium dxf - by Bioz Stars, 2026-10

94/100 stars

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1) Product Images from "Is there a pathological switch that triggers the onset of renal calcification?"

Article Title: Is there a pathological switch that triggers the onset of renal calcification?

Journal: bioRxiv

doi: 10.1101/2025.01.09.630316

Figure Legend Snippet: Study recap – recreating a pathological microenvironment on a kidney-on-a-chip microfluidic instrument to investigate the presence of a biological switch underlying renal calcification. A) Repurposing a proximal tubule-on-a-chip (kidney-on-a-chip) instrument for renal patient-derived macrophage M1 and M2 proliferation on the top and bottom channels of the organ-chip instrument. B) a-c: The confluence of the macrophage cell lines was well supported on the organ chip instrument surface and on the tissue-culture plates: the cluster-forming cells are motile. Apoptotic assays were conducted on a chip and a plate to emulate bioinspired oxidative stress to observe the expression of the biomarkers of prolyl hydroxylase domain 2 (PHD2) and CellRox dye (orange and green, respectively). The morphology of the cells dramatically changed from miniature, motile M1 and M2 variants to aggregated cell clumps emitting PHD2 and CellRox under hypoxia [Jeewandara T et al. 2023].

Techniques Used: Derivative Assay, Expressing

Using fluorescence-activated cell sorting (FACS) to a) distinguish M1 polarized macrophages stained with CD86 vs. the unstained M1 polarized macrophages, and b) distinguishing M2 polarized macrophages stained with CD163 vs. the unstained M2 polarized macrophages .

Figure Legend Snippet: Using fluorescence-activated cell sorting (FACS) to a) distinguish M1 polarized macrophages stained with CD86 vs. the unstained M1 polarized macrophages, and b) distinguishing M2 polarized macrophages stained with CD163 vs. the unstained M2 polarized macrophages .

Techniques Used: Fluorescence, FACS, Staining

Repurposing a human proximal tubule kidney chip to A) facilitate the growth of M1 pro- and M2 anti-inflammatory macrophages. B) The bright field images are representative of cell proliferation on the kidney chip vs. a 35 mm tissue culture plate [Emulate. Inc, ].

Figure Legend Snippet: Repurposing a human proximal tubule kidney chip to A) facilitate the growth of M1 pro- and M2 anti-inflammatory macrophages. B) The bright field images are representative of cell proliferation on the kidney chip vs. a 35 mm tissue culture plate [Emulate. Inc, ].

Techniques Used:

The apoptosis assays conducted with stone former M1 and M2 macrophages cultured on a plate (top) and on an organ-on-a-chip/kidney-chip instrument (bottom) incubated with CellRox (Redox) dye (green) prior to (left) - and after (right) chemically induced cell apoptosis .

Figure Legend Snippet: The apoptosis assays conducted with stone former M1 and M2 macrophages cultured on a plate (top) and on an organ-on-a-chip/kidney-chip instrument (bottom) incubated with CellRox (Redox) dye (green) prior to (left) - and after (right) chemically induced cell apoptosis .

Techniques Used: Cell Culture, Incubation

Apoptosis assays on a chip/plate (fluorescence imaging) – the M1 and M2 macrophage cell types were incubated with the AlexaFluor555 EGLN1/anti-PHD2/prolyl hydroxylase domain 2 antibody and subjected to chemical ischemia-induced redox (a-d) to observe the transition from healthy motile cells (left) to fluorescent clumps or aggregates (right) to indicate apoptosis or cell death under ischemia, represented on a 35 mm tissue culture plate and on a kidney-chip instrument. The fluorescence dye emission of AF555 represented a switch-like upregulation of PHD2 (circled in b), to glow in orange and emit a switch-like response as the cells underwent apoptosis under live-cell imaging (b,d).

Figure Legend Snippet: Apoptosis assays on a chip/plate (fluorescence imaging) – the M1 and M2 macrophage cell types were incubated with the AlexaFluor555 EGLN1/anti-PHD2/prolyl hydroxylase domain 2 antibody and subjected to chemical ischemia-induced redox (a-d) to observe the transition from healthy motile cells (left) to fluorescent clumps or aggregates (right) to indicate apoptosis or cell death under ischemia, represented on a 35 mm tissue culture plate and on a kidney-chip instrument. The fluorescence dye emission of AF555 represented a switch-like upregulation of PHD2 (circled in b), to glow in orange and emit a switch-like response as the cells underwent apoptosis under live-cell imaging (b,d).

Techniques Used: Fluorescence, Imaging, Incubation, Live Cell Imaging

A proposed model for the formation of Randall’s plaque in the presence of M1 pro-inflammatory and M2 anti-inflammatory phenotypes. Steps a-h recap the risk factors of hyperoxaluria, hypercalciuria, and hypocitraturia and other factors involved in the pathogenesis of nephrolithiasis to cause epithelial damage, the production of calcifying vesicles, increased pro-inflammatory macrophages vs. anti-inflammatory macrophages, collagen deposition, mineralization, and Randall’s plaque formation .

Figure Legend Snippet: A proposed model for the formation of Randall’s plaque in the presence of M1 pro-inflammatory and M2 anti-inflammatory phenotypes. Steps a-h recap the risk factors of hyperoxaluria, hypercalciuria, and hypocitraturia and other factors involved in the pathogenesis of nephrolithiasis to cause epithelial damage, the production of calcifying vesicles, increased pro-inflammatory macrophages vs. anti-inflammatory macrophages, collagen deposition, mineralization, and Randall’s plaque formation .

Techniques Used:

Incubation:

Article Title: Tumor-associated macrophages in direct contact with prostate cancer cells promote malignant proliferation and metastasis through NOTCH1 pathway.
Article Snippet: .. For PBMC-derived M2 macrophages, an appropriate amount of complete M2 macrophage Generation Medium DXF (Promo Cell, Heidelberg, Germany) was incubated with the cells according to the manufacturer's instructions. .. CD163 and CD206 expression in M2 macrophages was measured by flow cytometry using anti-CD163 and anti-CD206 antibodies (BD Biosciences, San Diego, CA, USA) according to the manufacturer’s instructions.

Article Title: Targeting the vascular endothelial growth factor receptor-1 by the monoclonal antibody D16F7 to increase the activity of immune checkpoint inhibitors against cutaneous melanoma.
Article Snippet: The vascular endothelial growth factor receptor-1 (VEGFR-1) is a membrane receptor for VEGF-A, placenta growth factor (PlGF) and VEGF-B that plays a crucial role in melanoma invasiveness, vasculogenic mimicry and tumor-associated angiogenesis.. Furthermore, activation of VEGFR-1 is involved in the mobilization of myeloid progenitors from the bone marrow that infiltrate the tumor.. Myeloid-derived suppressor cells and tumor-associated macrophages have also been involved in Jo ur na l P re -p ro of

Article Title: Tumor-associated macrophages in direct contact with prostate cancer cells promote malignant proliferation and metastasis through NOTCH1 pathway
Article Snippet: .. For PBMC-derived M2 macrophages, an appropriate amount of complete M2 macrophage Generation Medium DXF (Promo Cell, Heidelberg, Germany) was incubated with the cells according to the manufacturer's instructions. .. CD163 and CD206 expression in M2 macrophages was measured by flow cytometry using anti-CD163 and anti-CD206 antibodies (BD Biosciences, San Diego, CA, USA) according to the manufacturer's instructions.

Cell Culture:

Article Title: Lack of Mucosal Cholinergic Innervation Is Associated With Increased Risk of Enterocolitis in Hirschsprung’s Disease
Article Snippet: .. The attached monocytes were washed twice with PBS and then cultured for 10 days in M1 or M2 Macrophage Generation Medium DXF (PromoCell, Heidelberg, Germany) according to the manufacturer’s instructions. .. On day 10, cells were detached by adding Accutase cell detachment solution (Sigma-Aldrich), washed twice with RPMI, and used for further applications.

other:

Article Title: Biostimulating fillers and induction of inflammatory pathways: A preclinical investigation of macrophage response to calcium hydroxylapatite and poly-L lactic acid.
Article Snippet: M1- Macrophage Generation Medium DXF was used to culture M1 macrophages, and M2- Macrophage Generation Medium DXF was used to culture M2 macrophages (Promocell, Heidelberg, Germany).

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